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KMID : 0811720140180050509
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Korean Journal of Physiology & Pharmacology
2014 Volume.18 No. 5 p.509 ~ p.516
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Distinct Cellular Calcium Metabolism in Radiation-sensitive RKO Human Colorectal Cancer Cells
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Kim Yun-Tai
Jo Soo-Shin
Park Young-Jun
Lee Myung-Za
Suh Chang-Kook
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Abstract
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Radiation therapy for variety of human solid tumors utilizes mechanism of cell death after DNA damage caused by radiation. In response to DNA damage, cytochrome c was released from mitochondria by activation of pro-apoptotic Bcl-2 family proteins, and then elicits massive Ca2＋ release from the ER that lead to cell death. It was also suggested that irradiation may cause the deregulation of Ca2＋ homeostasis and trigger programmed cell death and regulate death specific enzymes. Thus, in this study, we investigated how cellular Ca2＋ metabolism in RKO cells, in comparison to radiation-resistant A549 cells, was altered by gamma (γ)-irradiation. In irradiated RKO cells, Ca2＋ influx via activation of NCX reverse mode was enhanced and a decline of [Ca2＋]i via forward mode was accelerated. The amount of Ca2＋ released from the ER in RKO cells by the activation of IP3 receptor was also enhanced by irradiation. An increase in [Ca2＋]i via SOCI was enhanced in irradiated RKO cells, while that in A549 cells was depressed. These results suggest that γ-irradiation elicits enhancement of cellular Ca2＋ metabolism in radiation-sensitive RKO cells yielding programmed cell death.
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KEYWORD
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A549 cells, Inositol-1, 4, 5-triphosphate receptors, Na＋-Ca2＋ exchanger, RKO cells, Store- operated Ca2＋ influx
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